Accumulation of actin, Arp2/3, vinculin, and WASP is talin-dependent. NK cells generated in vitro from wild-type (WT) or talin-knockout (KO) embryonic stem (ES) cells were incubated with ICAM-1 (IC-1) or PLL beads as indicated. Cells were fixed, permeabilized, and stained for actin (A), Arp2/3 (B), vinculin (C), or WASP (D). Cells were stained and analyzed by confocal microscopy as in Figure 1. The percent fluorescence intensity of staining at the contact site between an NK cell bound to an (▬) ICAM-1– or (▭) PLL-coated bead was calculated as in Figure 1 and shown in bar graphs to the right of confocal images. Error bars indicate SEM, n = 10 per condition. ***P < .005 compared with corresponding PLL condition.