Figure 3
Transplanted TGF-β1 deficient progenitors have decreased reconstitutive capacity with no sign of inflammatory/autoimmune disease. (A) Analysis of reconstitutive capacities of Lin− BM cells from 8- to 10-day-old TGF-β1+/+ and TGF-β1−/− mice. T-depleted Lin− cells (1 × 105, 2 × 105, 4 × 105, and 8 × 105) from male TGF-β1+/+ and TGF-β1−/− mice were transplanted into lethally irradiated CF-1 × Sv129 female mice (10 mice per group). Graphs represent the percentage of donor-derived cells (percentage of Y) in peripheral blood (PB) at 6 weeks (left) and 24 weeks (right) after transplantation (5 mice per group). An example of 2 independent experiments is shown (5 mice per dose of cells; *P < .05). The contribution of donor-derived cells is determined by quantifying chromosome Y sequence in PB of reconstituted irradiated mice in comparison to an autosomal sequence (titine). (B) Serum reactivity from mice that received a transplant with T-depleted Lin− BM cells from TGF-β1+/+ and TGF-β1−/− mice. Sera were collected at week 3 after transplantation. Reactivity was tested in an ANA stain as described by Oak et al39 on the human epithelial cell line Hep2. Antibody binding activity was shown with an anti–mouse immunoglobulin G FITC conjugate. Fluorescence was analyzed with an immunofluorescence microscope. MRL/lpr lupic mice were used as a positive control and TGF-β1+/+ mice as a negative control.

Transplanted TGF-β1 deficient progenitors have decreased reconstitutive capacity with no sign of inflammatory/autoimmune disease. (A) Analysis of reconstitutive capacities of Lin BM cells from 8- to 10-day-old TGF-β1+/+ and TGF-β1−/− mice. T-depleted Lin cells (1 × 105, 2 × 105, 4 × 105, and 8 × 105) from male TGF-β1+/+ and TGF-β1−/− mice were transplanted into lethally irradiated CF-1 × Sv129 female mice (10 mice per group). Graphs represent the percentage of donor-derived cells (percentage of Y) in peripheral blood (PB) at 6 weeks (left) and 24 weeks (right) after transplantation (5 mice per group). An example of 2 independent experiments is shown (5 mice per dose of cells; *P < .05). The contribution of donor-derived cells is determined by quantifying chromosome Y sequence in PB of reconstituted irradiated mice in comparison to an autosomal sequence (titine). (B) Serum reactivity from mice that received a transplant with T-depleted Lin BM cells from TGF-β1+/+ and TGF-β1−/− mice. Sera were collected at week 3 after transplantation. Reactivity was tested in an ANA stain as described by Oak et al39  on the human epithelial cell line Hep2. Antibody binding activity was shown with an anti–mouse immunoglobulin G FITC conjugate. Fluorescence was analyzed with an immunofluorescence microscope. MRL/lpr lupic mice were used as a positive control and TGF-β1+/+ mice as a negative control.

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