Immunologic status of 8- to 10-day-old TGF-β1^−/− mice. (A) Analysis of CD4⁺ CD25⁺ expression in CD3 T cell of thymus, lymph node, and spleen cells from 8- to 10-day-old TGF-β1^+/+ and TGF-β1^−/− mice. Histograms are the mean ± SD of 3 independent experiments, *P < .05. (B) Analysis of FOXP3 expression in CD4⁺CD25⁺ cells from spleen, lymph node, and thymus. The flow cytometric profiles are the representative histograms of 2 experiments (6 mice per experiment). (C) Analysis of the Lin⁻ Sca-1⁺ c-Kit⁺ (LSK) population in BM cells from 8- to 10-day-old TGF-β1^+/+ and TGF-β1^−/− mice. The flow cytometric profiles are representative of 3 independent experiments with 6 mice per experiment. (D) Analysis of the CD150⁺ CD48^low (signaling lymphocyte activation molecule) population in LSK BM cells from 8- to 10-day-old TGF-β1^+/+ and TGF-β1^−/− mice. The flow cytometric profiles are representative of 3 independent experiments with 6 mice per experiment, *P < .05. PE indicates phycoerythrin; Cy7, indocyanine 7.