PcG proteins are required for the serial replating capacity of Evi1-transduced BM cells. (A,C) Serial replating capacity of Evi1-expressing cells was markedly reduced with EZH2 (A), SUZ12, or EED (C) shRNAs (n = 8 from 4 independent experiments. Error bars indicate SD; *P < .01 and **P < .001; P values were calculated by comparisons with the colony numbers of control cells at the second replating. (B) RNAi of SUZ12 and EED. (D) A scheme showing that Evi1 recruits polycomb complexes to the PTEN promoter and represses PTEN transcription through H3K27me3-mediated chromatin remodeling, which, in turn, activates downstream AKT/mTOR pathway.