Trib1 and Trib2, but not Trib3, inhibit myeloid differentiation. The 32D cells were transduced with MigR1, Trib1, Trib2, or Trib3 and plated in IL-3 (A) or G-CSF (B) 48 hours after transduction. The number of GFP-expressing cells were determined at the indicated time points. Values are mean (± SD) for triplicate samples. (C) Flow cytometric analysis of CD11b expression, which indicates differentiation, is shown at day 3 after IL-3 or G-CSF treatment. Data are representative of 3 independent experiments.