Activation of MAPKs in response to hDectin-1 stimulation is impaired after PPAR-γ activation. DMSO- or TGZ-treated DCs were stimulated with curdlan for 4-24 hours and whole-cell lysates were prepared. The phosphorylation and thus activation of Syk (A) and p38 and ERK (B) were analyzed by Western blotting. The data shown are representative of at least 3 independent experiments.