PPAR-γ activation inhibits the hDectin-1–induced maturation in mDCs independently from hDectin-1 expression. mDCs were generated from adhering monocytes in the presence of the PPAR-γ ligand TGZ or DMSO as a control. Cells were stimulated with curdlan on day 6 of culture. (A) Expression of surface maturation markers was analyzed on day 7 by flow cytometry. Shown are the percentages of positive cells, along with mean fluorescence intensity values in square brackets. (B) The protein expression of hDectin-1 was analyzed on day 7 by Western blotting. The data shown are representative of at least 3 independent experiments.