Human Vδ2− γδ T-cell subsets display strong cytolytic responses against transformed B lymphoid target cells. (A) Cytolytic activity of 3 distinct mixed lymphocyte-tumor culture-derived human Vδ2− γδ T-cell clones (73R9, 73R14, and 84R33) was measured by 51Cr release assay after coculture together with Burkitt lymphoma (Daudi, Raji, and RJ225), multiple myeloma (RPMI226), B-LCL (Do#AD), and αβ T cells at both 20:1 and 5:1 γδ T cell-to-target ratios. (B) Cytolytic activity of a Vγ8Vδ3 T-cell clone (73R9) was measured by 51Cr release assay after coculture together with B cells from a panel of 7 B-LCLs generated from PBMCs of healthy donors (Do#) or 9 homozygous typing cells at a 20:1 γδ T cell-to-target ratio. (A–B) Data are the percentage of specific lysis in triplicate assays (mean ± SD), calculated as described in “Cytolytic activity.” Data are representative of at least 3 independent experiments performed with γδ T-cell clones from different healthy donors.
