Normal thrombin production in platelets lacking the GPIbα N-terminal domain. Washed platelets from WT mice (A) or washed human platelets (B) were left untreated (CTRL) or treated with Nk protease (Nk) or OSE until complete release of the GPIbα N-terminal domain (Tables 1–2). Thrombin generation was measured by the CAT method in platelets reconstituted in normal mouse plasma at 1.3 × 10⁸ cells/mL (A) or normal human plasma at 1 × 10⁸ cells/mL (B), after activation with TF (0.5pM) or CRP (10 μg/mL). ETP values are expressed as the mean ± SEM in 3 to 6 independent experiments.