DAC synergizes with SAHA in gene induction and cytotoxicity in Z138 cells. (A) Real-time PCR for mRNA expression of PCDH8, HOXD8, and MLF1 in Z138 cells before and after treatment with DAC 0.5μM daily for 3 days, SAHA 1μM (single dose on day 0), and their combination. Data are representative of 3 separate experiments. Error bars indicate SD. (B) Z138 cells were treated with DAC 0.5μM daily for 3 days and SAHA 1μM (single dose on day 0) alone, and in combination. Cell viability (y-axis) as measured by reduction of resazurin 48 hours after drug treatment (performed in 8 replicates).