American Society of Hematology

Figure 4

$Figure 4. Effects of B7-H1 molecules expressed by blasts on T cells. (A-C) Purified, normal CD3+, CD4+, and CD8+ T cells were cultured alone or with irradiated F-36P cells in the presence of either control immunoglobulin G (IgG), anti–B7-H1 monoclonal antibody, or anti–PD-1 monoclonal antibody. Data are mean (and SD) of 3 experiments. P < .05 when data in each of the 2 columns on the right were compared with control IgG data. (A) The percentage of annexin V+ cells in each T-cell fraction. (B) The percentage of caspase-3+ cells in T cells. Data labeled control IgG were defined as 100% in each experiment. (C) T-cell proliferation determined in the 3H-thymidine incorporation assay. (D) Normal CD3+ T cells were cultured with irradiated F-36P cells that had been purified into either B7-H1+ or B7-H1− cells.$

Effects of B7-H1 molecules expressed by blasts on T cells. (A-C) Purified, normal CD3⁺, CD4⁺, and CD8⁺ T cells were cultured alone or with irradiated F-36P cells in the presence of either control immunoglobulin G (IgG), anti–B7-H1 monoclonal antibody, or anti–PD-1 monoclonal antibody. Data are mean (and SD) of 3 experiments. P < .05 when data in each of the 2 columns on the right were compared with control IgG data. (A) The percentage of annexin V⁺ cells in each T-cell fraction. (B) The percentage of caspase-3⁺ cells in T cells. Data labeled control IgG were defined as 100% in each experiment. (C) T-cell proliferation determined in the ³H-thymidine incorporation assay. (D) Normal CD3⁺ T cells were cultured with irradiated F-36P cells that had been purified into either B7-H1⁺ or B7-H1⁻ cells.

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