Vaccine PGE₂ DCs have a high stimulatory capacity. (A) The profile of cytokines secreted by PBLs on contact with allogeneic cDCs, vaccine DCs, and vaccine PGE₂ DCs was measured by cytokine bead array. The graph represents the fold change in cytokine production of vaccine DCs and vaccine PGE₂ DCs relative to cDCs of 3 different donors. The table presents the mean concentration (picograms per milliliter) of each cytokine in absolute numbers for all conditions. (B) IFN-γ production by PBLs cocultured with vaccine DCs or vaccine PGE₂ DCs in the absence () or presence () of neutralizing anti–IL-12 antibody. The graph represents the mean ± SD from 2 experiments (with cells from different donors) of relative IFN-γ production compared with control vaccine DCs (100% corresponds to 48 ± 5 pg/mL). *P < .05. (C) KLH-specific proliferation of PBLs from a patient vaccinated with KLH-loaded DCs. PBLs were cocultured with autologous DCs matured with the cytokine cocktail, vaccines, or vaccines with PGE₂ with or without KLH. Proliferation was measured by incorporation of tritiated thymidine. The graph represents mean ± SEM counts per minute relative to cDCs + KLH (100% corresponds to 1201 ± 818 cpm) of 3 experiments with different donors, performed in triplicate. represents DCs loaded with KLH; and represents DCs without KLH. *P < .05. ***P < .001. ≠Significant difference (P < .05) with cDCs with KLH. #Significant difference (P < .05) with cDCs without KLH.