Vaccine PGE2 DCs have a high stimulatory capacity. (A) The profile of cytokines secreted by PBLs on contact with allogeneic cDCs, vaccine DCs, and vaccine PGE2 DCs was measured by cytokine bead array. The graph represents the fold change in cytokine production of vaccine DCs and vaccine PGE2 DCs relative to cDCs of 3 different donors. The table presents the mean concentration (picograms per milliliter) of each cytokine in absolute numbers for all conditions. (B) IFN-γ production by PBLs cocultured with vaccine DCs or vaccine PGE2 DCs in the absence () or presence (
) of neutralizing anti–IL-12 antibody. The graph represents the mean ± SD from 2 experiments (with cells from different donors) of relative IFN-γ production compared with control vaccine DCs (100% corresponds to 48 ± 5 pg/mL). *P < .05. (C) KLH-specific proliferation of PBLs from a patient vaccinated with KLH-loaded DCs. PBLs were cocultured with autologous DCs matured with the cytokine cocktail, vaccines, or vaccines with PGE2 with or without KLH. Proliferation was measured by incorporation of tritiated thymidine. The graph represents mean ± SEM counts per minute relative to cDCs + KLH (100% corresponds to 1201 ± 818 cpm) of 3 experiments with different donors, performed in triplicate.
represents DCs loaded with KLH; and
represents DCs without KLH. *P < .05. ***P < .001. ≠Significant difference (P < .05) with cDCs with KLH. #Significant difference (P < .05) with cDCs without KLH.