Platelets do not affect LEC viability, proliferation, or migration in vitro.(A) Overnight culture of platelets with LECs does not affect endothelial cell viability measured by nuclear uptake of propidium iodide. Control indicates cells not treated with propidium iodide. (B) Overnight culture of platelets with LECs does not affect endothelial cell proliferation measured by uptake of BrdU after a 2-hour pulse. Control indicates cells not treated with BrdU. (C) Culture of platelets with LECs does not alter migration in a scratch assay. N = 3 for each group. Error bars indicate SD.