RUNX1/ETOtr dimers lost replating capacity and in vivo leukemogenic function. (A) Replating potential of murine lin-depleted bone marrow cells expressing the indicated RUNX1/ETOtr (REtr) forms. Serial replating of bone marrow cells was performed in the presence of IL-3, IL-6, and stem cell factor. (B) Kaplan-Meier survival curves of C57/BL6 mice transplanted with lin-depleted bone marrow cells transduced with either REtr or REtr-m5. (C) Body weight, hematocrit, and white blood cell counts of mice transplanted with nontransduced or lin-depleted cells expressing either REtr or REtr. (D) FACS analysis of bone marrow cells obtained from a mouse transplanted with REtr-transduced cells. (E) Similar to panel D, but transplanted with REtr-m5 expressing cells. The percentage of cells in each quadrant is indicated.