Lung lymphocytes from NY1DD mice have higher A_2AR mRNA than congenic C57BL/6 controls. Live (DAPI⁻) CD45⁺ pulmonary cells were sorted based on surface antigen staining. (A) iNKT (CD1d-tetramer⁺, CD3⁺), NK (NKp46⁺, CD3⁻), and CD3⁺ T cells (CD1d-tetramer⁻, CD3⁺). Quantitative RT-PCR was used to measure A_2AR transcript levels in sorted populations of pulmonary lymphocytes compared with a housekeeper transcript (cyclophilin). (B) NY1DD mice have increased levels of A_2AR transcripts in iNKT cells and NK cells, but not in CD3⁺ tetramer⁻ T cells compared with C57BL/6 mice. (C) NY1DD iNKT and NK cells have increased A_2AR/cyclophilin mRNA ratios compared with NY1DD CD3⁺ tetramer⁻ T cells.