ONX 0912 triggers apoptosis in MM cells, associated with PARP cleavage and caspase activation. (A) MM cell lines were treated with ONX 0912 for 48 hours and analyzed for apoptosis using annexin V/PI staining assay. (B-C) MM.1S cells were treated with ONX 0912 at the indicated doses for 48 hours and harvested; whole-cell lysates were subjected to immunoblot analysis with anti-PARP, anti–caspase-3, anti–caspase-8, anti–caspase-9, or anti-GAPDH Abs. FL indicates full-length; CF denotes cleaved fragment. Blots shown are representative of 3 independent experiments.