Expression of inflammatory and neurochemical mediators in the spinal cords of male hBERK1 mice. (A-B) mRNA expression using semiquantitative reverse-transcribed polymerase chain reaction. GAPDH expression shows the loading control. The first lane indicates size markers. (B) Bar represents band density for each product relative to band density of GAPDH. (C-D) Expression of phosphorylated and total proteins (MAPK/ERK, p38 MAPK, and STAT3) determined using Western immunoblotting. Bar represents band density of individual phosphoprotein relative to its total protein. (B,D) Data are the mean ± SEM of 4 separate experiments. ■ represents hBERK1 mice; □, HbA-BERK mice. *P < .05.