BERK and hBERK1 mice show increased heat and cold hyperalgesia. PWL after application of a heat stimulus (A,D) and nocifensive behaviors on a cold plate at 4 ± 1°C (B-C,E-F) were recorded for each mouse. Shorter PWL (in seconds) in response to heat stimulus is indicative of increased thermal hyperalgesia. A higher PWF in a 2-minute period and lower PWL on a cold plate are indicative of increased sensitivity to cold-induced nociception. Data are the mean ± SEM from 4 to 6 mice with 3 observations per mouse. Significance of differences between the indicated conditions in each panel: *P < .05, **P < .01. (A) PWL after a heat stimulus in age- and sex-matched BERK and HbA-BERK mice. (B) PWF on a cold plate in age- and sex-matched BERK and HbA-BERK mice. (C) PWL on a cold plate in age- and sex-matched BERK and HbA-BERK mice. (D) PWL after a heat stimulus in 9-month-old hBERK1 and age-matched HbA-BERK mice. (E-F) PWF (E) and PWL (F) on a cold plate in 15-month-old hBERK1 and age-matched HbA-BERK mice. M indicates male; F, female.