Figure 7
FIP200 deletion led to increased mitochondrion mass and ROS in fetal hematopoietic cells. (A) Lysates from E14.5 liver of control or CKO mice were analyzed by Western blotting using anti-p62 (top) or anti-vinculin (bottom) antibodies. Asterisk on the right indicates the p62 band. (B-C) Relative mitochondrial mass (B) and ROS (C) of E14.5 fetal liver cells as measured by mean fluorescent intensity (MFI) of MitoTracker Green staining and DCF-DA staining, respectively. (D-E) Relative mitochondrial mass (D) and ROS (E) of myeloid cells from E14.5 fetal liver cells as measured by MFI of MitoTracker Green staining and DCF-DA staining, respectively. Representative FACS profiles of DCF-DA staining are shown on the right in panel E. (F-G) Relative mitochondrial mass (F) and ROS (G) of fetal HSCs from E14.5 embryos as measured by MFI of MitoTracker Green staining and DCF-DA staining, respectively. Representative FACS profiles of MitoTracker Green staining and DCF-DA staining are shown on the right.

FIP200 deletion led to increased mitochondrion mass and ROS in fetal hematopoietic cells. (A) Lysates from E14.5 liver of control or CKO mice were analyzed by Western blotting using anti-p62 (top) or anti-vinculin (bottom) antibodies. Asterisk on the right indicates the p62 band. (B-C) Relative mitochondrial mass (B) and ROS (C) of E14.5 fetal liver cells as measured by mean fluorescent intensity (MFI) of MitoTracker Green staining and DCF-DA staining, respectively. (D-E) Relative mitochondrial mass (D) and ROS (E) of myeloid cells from E14.5 fetal liver cells as measured by MFI of MitoTracker Green staining and DCF-DA staining, respectively. Representative FACS profiles of DCF-DA staining are shown on the right in panel E. (F-G) Relative mitochondrial mass (F) and ROS (G) of fetal HSCs from E14.5 embryos as measured by MFI of MitoTracker Green staining and DCF-DA staining, respectively. Representative FACS profiles of MitoTracker Green staining and DCF-DA staining are shown on the right.

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