Trib2 mutant design and expression. (A) Schematic of the Trib2 deletion mutants with the amino acid numbers indicated. (B) Expression of Trib2 mutant proteins. 293T cells were transfected with each myc-tagged mutant, and protein lysates were assessed for protein expression by Western blot and probed with the anti-myc antibody 9E10. ns indicates nonspecific binding by the antibody.