Antigen delivery to dermal dendritic cells by Tn-based targeting preferentially induces a Th2-like response. We injected BALB/c mice (2 or 3 per group) intradermally with PBS (□), glycosylated multiple antigenic glycopeptide:Tn3-poliovirus (PV; ○), nonglycosylated multiple antigenic peptides:PV (•), or (A) nonglycosylated multiple antigenic peptides:PV with 10 μg of unmethylated cytosine-phosphate-guanosine (B-C). Two weeks later, draining lymph node cells (A-B) or T cells purified from the spleen (C) were analyzed for antigen-specific T-cell responses after stimulation with multiple antigenic glycopeptide:Tn3-PV. Cytokine production was analyzed by the Luminex system (Luminex Corporation). Data are expressed as mean ± SD picograms per milliliter of duplicates. Representative results from 2-4 experiments are presented. ND indicates not done.