Analysis of APL arising from mCG-PR^2VR mice. (A) Analysis of leukemic mCG-PR^WT (n = 11) and mCG-PR^2VR (n = 29) mice for spleen weights, complete blood counts, and immunophenotyping by flow cytometry for Gr-1⁺/CD117⁺ blasts. (B) Immunophenotyping and (C) Wright-Giemsa staining of spleen cells from representative leukemic mCG-PR^WT and mCG-PR^2VR mice. (D-E) Western blotting of cell lysates from (D) bone marrow of preleukemic mice (E) spleen from leukemic mice. * represents a nonspecific band observed in the bone marrow extracts. Controls are a U937-PR9 cell line that contains the PR cDNA under the control of a Zinc (Zn²⁺) inducible promoter treated with 100μM ZnSO₄ for 1 hour.