Analysis of apoptosis-related gene expression induced by AICAR in CLL cells. (A) Cells were untreated (□) or treated (■) with 0.5mM AICAR for 24 hours. Cells were lysed, and the mRNA levels of the BCL-2 family members were analyzed by RT-MLPA. Data are mean ± SEM (n = 19). ***P < .005, AICAR-treated versus untreated cells. (B) Cells were preincubated without (Control) or with 0.2μM 5-iodotubercidin (I) for 30 minutes, and 0.5mM AICAR was added for 24 hours. Cells were lysed, and the mRNA levels of BIM, BNIP3, BNIP3L, HRK, MOAP1, NOXA, and PUMA were analyzed by RT-MLPA. Two representative samples are shown (n = 3).