Impaired production of Th2 cytokines in mediastinal lymph nodes of CCRL2^−/− mice. Lymph nodes single-cell suspensions were obtained 24 hours after the last aerosol administration. (A) Total cells/mouse and percentage of DCs in gated CD11c⁺MHCII⁺ cells. (B) Lymph node cells were cultured (0.5 × 10⁶ cells/0.2 mL/well) with OVA for 96 hours. (C) WT or CCRL2^−/− OVA-pulsed bone marrow DCs (10⁶ cells/mouse) were injected intratracheally into WT mice at days 18 and 20. Forty-eight hours later, lymph nodes were excised and cytokine production determined after ex vivo stimulation with OVA. Data are mean ± SEM of 10-20 mice/group from > 2 separate experiments. *P < .05; **P < .01 in comparison to WT mice. # indicates undetectable levels.