Figure 2
Figure 2. Generation of CCRL2 deficient mice. (A) Exon/intron structure of the CCRL2 gene. (B) Schematic representation of WT and KO alleles of the CCRL2 gene and targeting vector. Restriction sites: K, KpnI; S, SalI; H, HindIII; B, BamHI; Sa, SacI; Sc, ScaI. KpnI-digested genomic DNA fragments were detected by a specific probe. (C) Southern blot analysis of KpnI-digested genomic DNA from transfected embryonic stem cells hybridized with the CCRL2 probe generated a 12-kb WT restriction fragment and a 3.5-kb homologous recombinant restriction fragment. (D) PCR analysis of genomic DNA of CCRL2+/+ (lane 1), CCRL2−/− (lanes 2 and 3), and CCRL2+/− (lane 4) mice. (E) Total RNA was isolated from immature as well as TNF-α– and LPS-stimulated (24 hours) bone marrow DCs.

Generation of CCRL2 deficient mice. (A) Exon/intron structure of the CCRL2 gene. (B) Schematic representation of WT and KO alleles of the CCRL2 gene and targeting vector. Restriction sites: K, KpnI; S, SalI; H, HindIII; B, BamHI; Sa, SacI; Sc, ScaI. KpnI-digested genomic DNA fragments were detected by a specific probe. (C) Southern blot analysis of KpnI-digested genomic DNA from transfected embryonic stem cells hybridized with the CCRL2 probe generated a 12-kb WT restriction fragment and a 3.5-kb homologous recombinant restriction fragment. (D) PCR analysis of genomic DNA of CCRL2+/+ (lane 1), CCRL2−/− (lanes 2 and 3), and CCRL2+/− (lane 4) mice. (E) Total RNA was isolated from immature as well as TNF-α– and LPS-stimulated (24 hours) bone marrow DCs.

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