Unaltered JAQ1-induced GPVI shedding in Adam10^−/−/Adam17^ex/ex double mutant mice. Control and Adam10^−/−/Adam17^ex/ex BMc mice were injected intravenously with 100 μg JAQ1^biotin. Flow cytometric analysis of platelet count (A) and GPVI expression (B; indirectly: streptavidin-FITC) was performed. As a positive control in (B), wild-type platelets from untreated mice were incubated with 10 μg/mL biotinylated-JAQ1 and stained with streptavidin-FITC. (C) Mice were injected with 100 μg biotinylated-JAQ1, and plasma was collected at the indicated time points. GPVI levels in plasma were determined by ELISA. Results of all experiments are mean ± SD (n = 4 mice per group, representative for 3 individual experiments).