Inactivation of hepatocyte-derived Hif-2 in P3Pro mutants blunts erythropoietic responses to hypoxia. (A) Hemoglobin concentrations (n = 23 for P3Pro; n = 11 for Alb/P3Pro) and serum Epo levels in Alb/P3Pro double mutants compared with P3Pro mutants (n = 4) under baseline conditions. (B) Serum Epo levels in P3Pro and Alb/P3Pro mutants after phlebotomy of (n = 7). Epo mRNA levels in livers of P3Pro and Alb/P3Pro mutants at baseline conditions (n = 3) and after phlebotomy (mice were analyzed 18 hours after phlebotomy; n = 7), as determined by real-time PCR. (C) Serum Epo levels in P3Pro (n = 13) and Alb/P3Pro mutants (n = 3) after chronic hypoxia exposure (10% O2 for 10 days) and corresponding Epo mRNA levels in P3Pro and Alb/P3Pro livers (n = 3). *P < .05; ns, not statistically significant.
