Bcl-2 and CFLAR/c-flip are essential to protect pDCs from GC-induced apoptosis. (A-B) pDCs stimulated overnight by Flu were exposed to GC in the presence of several siRNA targeting proapoptotic (BAX) or antiapoptotic (c-flip, Bcl-2, iap-2, Bcl-xL) molecules or scramble control siRNA (SCR). (C-D) pDCs stimulated overnight by Flu with GC in the presence of several siRNA targeting proapoptotic (caspase-8) or antiapoptotic (Bcl-2) proteins or SCR. Dot plots show apoptosis assessed using annexin V/PI staining of representative experiment (A,C). Histograms show the results obtained with 7 independent experiments (B,D). In all panels, percentage indicates the live cells in each condition. P values were determined using 2-tailed Student t test (***P < .001).