Figure 2
TLR triggering protects pDCs from GC-induced apoptosis. (A-B) Viability of pDCs cultured overnight with IL-3 (10 ng/mL) and GC at 10−4M in the presence of TLR-7/-9 ligands (Flu, Sendai virus, HSV, SAC, CpG-C26) and TLR-2/-3/-4 ligands (LPS, poly I:C, LTA, PGN). (C-E) Viability of pDCs cultured overnight with various GC concentrations (10−4 to 10−6M) in the presence of TLR-7/-9 stimulators (Flu, Sendai virus, CpG-C). (F) Viability of pDCs cultured overnight with GC (10−4 and 10−6M) in the presence of different CpG types (CpG-A, -B, and -C). Histograms represent the mean ± SD of at least 3 independent experiments. P values were determined using 2-tailed Student t test (NS, not significant; *P < .05; **P < .01; ***P < .001).
