Synectin is expressed in lymphatic vessels in zebrafish embryos. Scale bars represent 10 μm in panels A-F. In all panels, the dorsal side of the embryo is at the top of the figure. G indicates gut; NT, neural tube; P, pronephric duct; and S, somite. (A-C) Transverse sections through the trunk of a 3-dpf wild-type Fli1:eGFP^y1 zebrafish embryo, stained by immunohistochemistry using a polyclonal anti–human synectin antibody, and by DAPI nuclear stain, revealing prominent synectin expression in the NT, G, S, dorsal aorta (DA, white arrow), posterior cardinal vein (PCV, green arrow) and parachordal lymphangioblast (PL) cells (white arrowhead). Insets show magnification of the boxed areas (PL cells) in each panel. (D-F) Using the same staining procedure on transverse sections through the rostral trunk of a 7 dpf wild-type Fli1:eGFP^y1 zebrafish embryo, similar widespread synectin expression in the NT, G, S, P, DA (white arrow), PCV (green arrow), and thoracic duct (white arrowhead) was observed.