PDGF and VEGF are elevated in CLL plasma versus normal plasma. (A) RTK array analysis of the CLL MSC stimulated by either pooled CLL or normal plasma. (B) Densitometry analysis revealed that PDGFR activation was more evident in CLL MSCs when they were exposed to CLL plasma versus normal plasma. (C) Plasma PDGF levels in CLL patients were significantly elevated compared with normal subjects (CLL plasma, n = 38; mean ± SEM, 3121.0 ± 478.9 pg/mL; normal plasma, n = 11, 554.4 ± 88.6 pg/mL; P < .0001). Similarly, significantly elevated VEGF levels were found in CLL plasma compared with normal plasma (CLL plasma, n = 36, 117.1 ± 22.2 pg/mL; normal plasma, n = 9, 31.0 ± 3.1 pg/mL; P = .0007). The characteristics of the patients used in this study were presented in supplemental Table 1. The horizontal line in each dot plot represents the mean level of the tested growth factor.