Stat6-regulated gene expression profile of macrophages. (A) RNA was isolated from F4/80⁺-sorted (purity 99%), untreated (control), or IL-4–treated (10 ng/mL) wild-type (WT) and Stat6^−/− BMDM. Gene expression profile was performed on high-density oligonucleotide arrays (Affymetrix). (B) Quantitative RT-PCR analysis of selected genes that appeared differently expressed in untreated (black bars) or IL-4–treated (gray bars) WT and Stat6^−/− BMDM based on the microarray experiment. Samples were normalized to β-actin expression. The microarray data have been deposited to the Gene Expression Omnibus database under the accession number GSE20030.