NKAP deletion in Mx1-cre NKAP cKO mice results in lethality and hematopoietic failure. (A) Kaplan-Meier survival curve data of WT (n = 5) and Mx1-cre NKAP cKO (n = 5) treated with poly-IC. (B) Mx-1 cre NKAP cKO and a wild-type littermate were injected intraperitoneally with 200 μg of poly-IC at day 0, 2 and 4. At day 8, HSCs in the bone marrow were analyzed by flow cytometry. Shown is representative data from 3 separate Mx1-cre and wild-type littermate pairs. (C) Schematic representation of the experimental design to generate WT and Mx1-cre NKAP cKO radiation chimeras. (D) Kaplan-Meier survival curve data of WT (n = 4) and Mx1-cre NKAP cKO (n = 4) radiation chimeras treated with poly-IC. (E) CBCs with differential at day 14 (WT chimeras) or at killing when moribund (Mx1-cre NKAP cKO chimeras). Results are from 4 animals in each group. In all of the parameters shown, the difference between WT and vav-cre NKAP cKO pups using the Student t test is significantly significant (P < .002). Data are shown as the average ± SEM. (F) H&E staining of fixed femur sections from a WT and a moribund Mx1-cre NKAP cKO at day 12 after poly-IC induction.