Figure 3
BMDCs treated with LPS+ATPγS increased LLC tumor growth. (A) Coinjection of LLC cells and LPS+ATPγS-treated BMDCs increased tumor growth. BMDCs (5 × 105) were treated for 24 hours with PBS (CTRL), ATPγS (100μM), LPS (100 ng/mL), or LPS (100 ng/mL) plus ATPγS (100μM) and then incubated or not with an anti–mouse AREG blocking antibody for 3 hours. Treated BMDCs were coinjected with LLC cells (2.5 × 105), and LLC tumors were weighted at day 14. Results represent the mean ± SEM of 9 tumors at least for each condition. The Student t test was performed using Prism 5.0 software (*P < .05; **P < .01). (B) Pictures of LLC tumors 14 days after coinjection. Camera used to take pictures was a Nikon D70 with a Nikkor objective macro 60 mm.
