Mouse AREG is released by BMDCs. (A) AREG release by murine BMDCs. BMDCs were stimulated by ATP (300μM), ATPγS (100μM), UTP (100μM), PGE2 (500nM), or Ado (10μM) in the absence or the presence of LPS (100 ng/mL) for 24 hours. Supernatants of treated DCs were collected for ELISA measurements of mouse AREG. Results represent the mean ± SEM of 6 independent experiments at least. The Student t test was performed using Prism 5.0 software (*P < .05; ***P < .001). (B) Effect of NECA and MRS1754 on AREG secretion by BMDCs. BMDCs were stimulated in the presence of LPS (100 ng/mL), by ATP (300μM), ATPγS (100μM), or NECA (1μM) in the absence or the presence of MRS1754 (4μM) for 24 hours. Supernatants of treated DCs were collected for ELISA measurements of mouse AREG. Results represent the mean ± SEM of at least 3 independent experiments. The Student t test was performed using Prism 5.0 software (**P < .01; ***P < .001; n.s., not significant). (C) LLC growth is increased by AREG secreted by BMDCs. BMDCs were stimulated by ATPγS (100μM) in presence of LPS (100 ng/mL) for 24 hours. Supernatants were treated or not with an anti–mouse AREG blocking antibody during 3 hours and incubated with LLC cells for 24 hours. LLC cells were then counted with hemocytometer. Data (mean ± SEM) are representative of 3 independent experiments. The Student t test was performed using Prism 5.0 software (**P < .01).
