Endosteal, but not central, CD150⁺CD48⁺LSK have multilineage engraftment potential. (A) Endosteal and central CD150⁺CD48⁺LSK were transplanted in a competitive limiting dilution assay. (B) Survival of lethally irradiated recipients. (C) Graph shows percentage of progeny of endosteal (●) and central (○) CD150⁺CD48⁺LSK. (D) Graph shows lymphoid and myeloid progeny of endosteal CD150⁺CD48⁺LSK: > 1:300 endosteal CD150⁺CD48⁺LSK show physiologic multilineage reconstitution. (E) Graph shows lymphoid (red and purple circles) and myeloid (green circles) progeny of central CD150⁺CD48⁺LSK: Central CD150⁺CD48⁺LSK exhibit low levels of lymphoid but are devoid of myeloid progeny. Each dot represents individual recipients.