Endosteal and central CD150⁺CD48⁻LSK reconstitute and out-compete CD150⁺CD48⁺LSK. (A) Endosteal and central CD150⁺CD48⁻LSK were transplanted in a competitive limiting dilution assay with CD150⁺CD48⁺LSK from the same BM region. (B) Survival of lethally irradiated recipients. (C) Graph shows percentage of BM reconstitution from CD150⁺CD48^+/−LSK from endosteal and (D) central BM. (E) Plot shows lymphoid and myeloid progeny of endosteal and (F) central CD150⁺CD48⁻LSK. Both transplanted cell populations show physiologic multilineage reconstitution after 20 weeks. (G) Plot shows lymphoid and myeloid progeny of endosteal and (H) central CD150⁺CD48⁺LSK. Each dot represents individual recipients from 2 independent transplantations.