Role of CD48 in HSPC homing. (A) Representative analysis of CD41 (R2) and CD48 (R3) expression on endosteal and central LSK. Black represents isotype control; red, endosteal LSK; and blue, central LSK. (B) Unstained endosteal and central LSK were cotransplanted with endosteal and central LSK, labeled with CD48 (HM48.1 clone), CD48 (OX-78 clone), or CD41 antibody but not sorted on the basis of their expression, and the homing efficiency analyzed 15 hours after transplantation into nonablated and (C) ablated recipients. (D) Spatial distribution of LSK or LSK stained with CD48 (HM48.1) 15 hours after transplantation into nonablated and ablated (E) recipients. (B-C) Dot graphs: Each dot represents an individual recipient; line represents the mean. (D-E) Bar graphs: At least 50 cells from 6 sections from 3 individual recipients were analyzed. Data are mean ± SEM.