Blebbistatin and nocodazole do not block enucleation of primary mouse fetal liver erythroblasts. (A) Schematic of 48-hour fetal liver erythroblast enucleation assays. Typically, this system results in the accumulation of 15%-30% of enucleated cells. (B) Flow cytometric analysis of enucleation, cell cycle, and cell death of fetal liver cultures supplemented with blebbistatin or nocodazole at 24 or 38 hours. All flow cytometric analyses were performed after 48 hours of total culture time, and the profiles were shown. The percentage of reticulocytes after incubation were quantified by Syto 64/Ter119 staining, cell-cycle status by propidium iodide, cell death by annexin V/SytoX red, and differentiation status by Ter119 positivity.