Loss of Cdc73 leads to decreased proliferation of leukemia cells. (A) Western blotting was performed on whole cell lysate for the MA-Cdc73fl-CreER and EH-Cdc73fl-CreER cell lines treated with EtOH or 5 nM 4-OHT for 48 hours. Cdc73 protein is reduced after 48 hours of 4-OHT treatment. β-actin serves as a loading control. (B-C) MA-Cdc73fl-CreER, EH-Cdc73fl-CreER, and control cell lines (MA-CreER, EH-CreER) were grown in liquid culture in the presence of either EtOH or 5 nM 4-OHT and counted daily for 5 days. The total cell number for each day is plotted for each condition (mean ± standard deviation [SD]; n = 2 independent experiments; NS = not significant (P > .05); 2-way analysis of variance [ANOVA]). (D-E) qRT-PCR performed on either the MA-Cdc73fl-CreER and MA-CreER or EH-Cdc73fl-CreER and EH-CreER cell lines 48 hours after EtOH or 4-OHT treatment. Expression levels for Hoxa9, Meis1, and Bcl2 are shown relative to each cell line treated with EtOH (mean ± SD; n = 2 independent experiments. ****P < .001; **P < .005; *P < .05; NS, not significant (P > .05); unpaired 2-tailed Student t test).