Subcellular localization of transduced TAT-NF-Ya in different types of (A-B) Human peripheral blood cells, (C) NIH 3T3 cells, (D) K562 cells. Cells treated with TAT-NF-Ya were incubated with anti-GST AlexaFluor 488 conjugate. (A) Antibody-stained control cells that were not transduced; 2 areas of a single slide are shown. (B,D) Arrows indicate examples of nuclear staining. (D) Inset: Magnified image of the cell denoted by the arrow. (A-B,D) Bar represents 20 μm. (C,D inset) Bar represents 10 μm. Procedures for protein delivery and visualization are described in “TAT-NF-Ya protein delivery to the cells.”