Analysis of the expression of NADPH oxidase in neutrophils of human G6PC3-deficient patients. Annexin V–depleted blood neutrophils were isolated from HD (n = 5; ages 23 to 37 years) and 2 G6PC3-deficient patients, P1 and P2 as described in “Depletion of apoptotic cells from neutrophils.” Two separate experiments were conducted, each analyzed in duplicates. (A) Representative immunofluorescence analysis of gp91^phox (red fluorescence) and DAPI nuclei staining (blue fluorescence) at magnifications of 630×. (B) Representative immunofluorescence analysis of p47^phox (green fluorescence), pan Cadherin membrane staining (red fluorescence), and DAPI nuclei staining (blue fluorescence) at 630× magnification.