Figure 5
VAP-1 activity is essential at the early steps of metastasis but dispensable afterward. BALB/c or C57BL/6 mice were treated with VAP-1 inhibitor either 1 hour before and 24 hours after the intravenous injection of 5 × 105 4T1-GFP (left) or B16F10 (right) tumor cells, respectively, (VAP-1 inhibition early), or on days 5, 7, 9, and 11 after the introduction of the tumor cells (VAP-1 inhibition late). Lungs were isolated on day 14 and the number of metastatic lung nodules was analyzed. Data represent mean + SD; n ≥ 10 mice (one-way analysis of variance and Tukey test) and **P < .01; ***P < .001.
