Figure 1
![Figure 1. Deregulation of Wnt pathway gene expression in CLL. (A) Detection of LEF1, WNT3, TCF4 [ITF-2], and CCND2 mRNA levels by quantitative PCR in leukemic or normal B cells from 8 CLL patients and 3 healthy donor samples, respectively. Transcript levels were normalized to 18s rRNA, and relative fold change was calculated using the 2−ΔΔCT method. *P < .05. Error bars represent SEM. (B) Detection of IGFBP-4 protein secretion into culture media by ELISA in B cells from 6 CLL patients and 3 healthy donor samples. The dashed line at 5 ng/mL indicates the ELISA threshold level of IGFBP-4 detection, and the horizontal lines indicate the mean of each group.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/16/10.1182_blood-2010-02-269878/4/zh89991058320001.jpeg?Expires=1722703267&Signature=hIfwcI-Um76vRvZ42AcJhEyxs-H5ntTayFMuUP01kdQMDY6aHf3utNPeNUsV~7W84-lCWSqyme0T7qDXObYFBx1eyHy35V9CGiWALCCxxXbLkbPZ2QlWJBGZNW8xpnFCP~gfs0nKSN7mPBQzt-wB9zhlj41as0nJWC8lxEibHtTJYorvHVJdZNdQQ5mK3wDW0~VBozexSRSNJBRmJiIe0VJ~dg2FS2531n8caYt1ulEQXurvOMJfMEgMkVnJwpUFCxkwXLOmDP8hiLmHYAEzLnfUt9Ab5oZd9SarQpwHafTflm7lgqbR51hrFLRg1qbweFuqgOWfMCqF~H5KgxpLVQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Deregulation of Wnt pathway gene expression in CLL. (A) Detection of LEF1, WNT3, TCF4 [ITF-2], and CCND2 mRNA levels by quantitative PCR in leukemic or normal B cells from 8 CLL patients and 3 healthy donor samples, respectively. Transcript levels were normalized to 18s rRNA, and relative fold change was calculated using the 2−ΔΔCT method. *P < .05. Error bars represent SEM. (B) Detection of IGFBP-4 protein secretion into culture media by ELISA in B cells from 6 CLL patients and 3 healthy donor samples. The dashed line at 5 ng/mL indicates the ELISA threshold level of IGFBP-4 detection, and the horizontal lines indicate the mean of each group.
