Figure 4
Increased gata1 expression in wild-type and cdx4−/− embryos by blocking RA with the use of DEAB or AGN193109. Each panel shows representative embryos from one experiment. Comparable results were obtained in at least 3 replicate experiments. (A-D) Whole-mount in situ hybridization for gata1 and myoD expression in flat-mounted embryos 14 to 15 ss treated with DMSO control or DEAB (10−5M) to block RA biosynthesis. Embryos were treated with DEAB from 60% epiboly until 14 to 15 ss. (E-J) Whole-mount in situ hybridization for gata1 and myoD expression in flat-mounted embryos at 10 to 11 ss treated with DMSO control, DEAB (10−5M), or AGN193109 (2 × 10−5M), a pan-RAR antagonist. Embryos were treated from 60% epiboly until 10 ss, then fixed.
