Fibrinogen UTR gene reporter assays to detect direct effects of hsa-miR-29 family miRNAs. (A) The relative luciferase activity normalized with a nontransfected condition. Firefly luciferase reporter gene plasmids containing the fibrinogen 3′-UTRs (FGA, FGA-αE, FGB, FGG, and FGG′) were individually cotransfected with the hsa-miR-29c precursor molecule (10, 30, and 90nM) and a renilla luciferase transfection control plasmid in HEK-293T cells; n = 3. (B) A similar experiment using a reporter plasmid containing the FGG 5′-UTR sequence cloned upstream of the firefly luciferase reporter gene or a control plasmid without the FGG 5′-UTR cotransfected with 10nM or 30nM miRNA precursor molecules. P values were calculated for experimental data versus values for control plasmid conditions: *P < .05, **P < .01, ***P < .001 (2-sided t test). Error bars represent SD; n = 3. ns indicates not significant.