Down-regulation of fibrinogen production by hsa-miR-29 family members. (A) The normalized secreted fibrinogen (Fbg) levels for hsa-miR-29a, hsa-miR-29b, and hsa-miR-29c. Precursors were transfected at 3 concentrations (3.3, 10, and 30nM, gray bars), and secreted fibrinogen was analyzed. A negative control miRNA precursor (Neg. Ctrl.) and hsa-miR-144, which had no effect on fibrinogen production in the screen, were used as negative controls (▭). A nontransfected control was used as the normalizing condition (▬). P values were calculated for experimental data versus values for Neg. Ctrl. (B) Relative steady-state levels of each of the 5 fibrinogen transcripts (FGA, FGA-αE, FGB, FGG, and FGG′) were quantified by quantitative RT-PCR from RNA samples of HuH7 cells transfected with 30nM hsa-miR-29. hsa-miR-144 was used as a negative control. P values were calculated for experimental data versus values for hsa-miR-144: *P < .05, **P < .01, ***P < .001 (2-sided t test). Error bars represent SD; n = 3. ns indicates not significant.