Fibrinogen regulatory potential of 470 precursor miRNA molecules in vitro. The frequency distribution of 470 miRNA precursors is plotted for fibrinogen production fold change on the x-axis (log₂). The gray area represents miRNAs that are considered to have no effect on fibrinogen production, using a threshold of at least 25% decrease or increase. Each precursor molecule was tested at 30nM in 3 independent transfection experiments, using HuH7 cells. Fold change in fibrinogen production was calculated relative to a negative control miRNA precursor used to normalize data.