Suppression of NF-E2 expression by siRNA-mediated inhibition of AML1 and CBF-β expression. UKE-1 cells were nucleofected with the indicated siRNAs or mock treated (MOCK). Seventy-two to 96 hours after nucleofection, protein extracts were prepared and analyzed by Western blotting for (A) NF-E2, AML1, and β-actin expression or (C) NF-E2, CBF-β, and β-actin expression. (B and D) Quantification and statistical analysis of NF-E2 protein expression in at least 3 independent experiments, representative examples of which are shown in panels A and C, respectively. NF-E2 expression in negative control 2 treated cells was set at 100%. *P < .05 by t test.