Activation of AIM2 inflammasome is not regulated by PML. (A) Normal AIM2-mediated IL-1β production in PML-deficient macrophages. Pml^+/+ and Pml^−/− BMDMs were primed with LPS for 3 hours, followed by nigericin treatment for 4 hours or dAdT for 6 hours, and the amount of IL-1β secreted was quantitated. dAdT (1.5 μg) was transfected using Lipofectamine 2000. (B) PML-I did not increase the activation of the reconstituted AIM2 inflammasome. 293T cells were transfected with the indicated components of AIM2/NLRP3 inflammasome, with or without PML-I. Empty vector was included to ensure the same amount of DNA was transfected in each set. The production of IL-1β was determined 48 hours later. Experiments were independently repeated 3 times. **P < .01; ***P < .001, for paired t test.